Fife Council is urging people in the Kingdom to back the Levenmouth Rail Campaign.
The area is the most populated in Scotland (37,000) not to be serviced by a rail link.
Calls to reactivate the five mile line between Leven and Thornton, which closed to passengers in 1969, have intensified with the growth of distillery firm Diageo and the establishment of Fife Energy Park in Methil.
The move would cost £55million, while the ongoing Borders railway project is set to amount to £300 million.
Allen Armstrong, secretary of Levenmouth Rail Campaign said: "There's a great opportunity emerging now for the Leven rail-link to be the next main rail re-connection in Scotland after the success of Airdrie-Bathgate, Stirling-Alloa and soon the Borders lines.
"We appreciate Fife Council's backing on this key issue for Levenmouth, including helping produce this leaflet.
"The Feasibility Study will be ready by June 2015 but the strong economic case it will make needs to be bolstered by stronger local support and pressure. If local people want to catch the train from Leven after a gap of 45 years it's up to them to show their support to national decision-makers by signing the petition, getting busy on social media and pestering their elected representatives."
Deputy Leader Cllr Lesley Laird said: "Fife Council is committed to this project. Re-opening the Levenmouth Rail Link is vital for improving the local economy in the area.
"Levenmouth has so much to offer residents, businesses and tourists. There is no doubt, improving its transportation links would open up the potential of the area and its people to do even more."