Amber Drysdale, who's 14 years old, was last seen on Tuesday afternoon in the Links Street area of Kirkcaldy.
Police say she has strong links to the Levenmouth area. Officers say they're growing increasingly concerned for Amber's welfare.
The teenager is described as a white teenage girl. She is around 5ft 6” in height with blue eyes and fair hair shaven on one side. She has a facial piercing and ‘spacer’ in her right ear. She was last seen wearing a bright yellow Adidas top and New York baseball cap.
Inspector Tom Brown said: “Amber has been missing since Tuesday yesterday and we are growing increasingly concerned for her welfare.
“I’d urge anyone who may have seen her since yesterday afternoon, or has information which can help with our enquiries, to come forward.”
Anyone with information is asked to contact Police Scotland as soon as possible on 101 or anonymously through Crimestoppers on 0800 555 111.