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Work at the Bothwell Gardens Roundabout in Dunfermline resumes on Sunday.
Fife Council's upgrading traffic signals and resurfacing between Elgin Street, Moodie Street and Nethertown Broad Street.
All work after Sunday will run 7pm to 6am.
It's in a bid to minimise disruption to daytime traffic, with work earlier this month causing lengthy queues.
This final phase will last for three weeks.
Fife Council's service manager for roads and lighting Mark Dewar said: "We understand how disruptive the earlier phases of these roadworks were and we're now taking a new approach to minimise further disruption.
"We thank road users for their continued patience and cooperation as we get Dunfermline ready for future developments."
The local authority say these road improvements are "necessary" to allow new development around the city.
The planned growth in Dunfermline, which includes 8,000 new homes, four new primary schools and development of employment land in the coming years, it says means it "needs to act now" to ensure traffic can continue to move freely.
A council spokesperson added: "These current road improvements are part of the transport measures identified as necessary to help mitigate the impacts of the planned development and follow on from the new traffic lights which were installed at the A823 Pitreavie roundabout in 2022, which were the first phase of these developer funded measures."