A total of 33 people in Fife have now tested positive for the April-02 variant of coronavirus - or the so-called Indian variant - a rise of 25 from the last update.
NHS Fife says that while the majority of the affected patients live in Kirkcaldy, a small number are known to live in other parts of the Kingdom.
It says "intensive" work is taking place to identify those who have been in close contact with the positive cases in order to tell them to isolate.
The variant is thought to be more transmissible than others in circulation.
The vast majority of cases are among those living in the Smeaton, Hayfield, Bennochy, Valley and Templehall areas of Kirkcaldy - and people living in these areas continue to be urged to get tested as soon as possible.
Drop-in centres are operating at the YMCA car park on Hendry Crescent, Victoria Road car park, and at the Glebe Park Neighbourhood Centre, where home testing kits can also be collected.
People with symptoms can continue to book tests at Overton Road.
Dr Lorna Wilson, consultant in public health medicine, said: "The overall levels of Covid-19 in Fife remain stable currently, however, we have seen an increase in the proportion of those being confirmed cases of the B.1.617.2 variant over the last week or so. The variant is being monitored very closely across Scotland.
"The B.1.617.2 variant is known to be more infectious. Testing in the community is a vital part of preventing the virus from spreading and we need as many people as possible, particularly those living in the affected parts of Kirkcaldy, to visit one of the many community testing facilities available.
"Getting tested is free, you don’t need an appointment and you could potentially help prevent many more people becoming unwell with the virus.
"With the overall cases of Covid-19 having reduced over recent months, and the gradual easing of restrictions, it is easy to think that Covid-19 is no longer a threat but that could not be further from the truth.
"We must all remain vigilant and if we develop any of the symptoms of Covid-19 regardless of how mild, isolate immediately and arrange to get tested as soon as possible.
"It is also important that individuals take up the offer of vaccination when invited and ensure that they get both doses."
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